Electromechanical Unzipping of Individual DNA Molecules Using Synthetic Sub-2 nm Pores
نویسندگان
چکیده
منابع مشابه
Electromechanical unzipping of individual DNA molecules using synthetic sub-2 nm pores.
Nanopores have recently emerged as high-throughput tools for probing and manipulating nucleic acid secondary structure at the single-molecule level. While most studies to date have utilized protein pores embedded in lipid bilayers, solid-state nanopores offer many practical advantages which greatly expand the range of applications in life sciences and biotechnology. Using sub-2 nm solid-state n...
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We have used the nanometer scale alpha-Hemolysin pore to study the unzipping kinetics of individual DNA hairpins under constant force or constant loading rate. Using a dynamic voltage control method, the entry rate of polynucleotides into the pore and the voltage pattern applied to induce hairpin unzipping are independently set. Thus, hundreds of unzipping events can be tested in a short period...
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Biological pores regulate the cellular traffic of a large variety of solutes, often with high selectivity and fast flow rates. These pores share several common structural features: the inner surface of the pore is frequently lined with hydrophobic residues, and the selectivity filter regions often contain charged functional groups. Hydrophobic, narrow-diameter carbon nanotubes can provide a sim...
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At some time almost 4 billion years ago, nature likely was faced with a chemical dilemma. Nucleic acids had emerged as replicable information carriers and primitive catalysts (Joyce 2002), yet their functional potential was constrained by their structural homogeneity and lack of reactive groups. These properties rendered nucleic acids well suited for storing information, but fl awed for mediati...
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We study theoretically the mechanical failure of a simple model of double-stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder model of shear unzipping proposed earlier by deGennes [de Gennes, P. G. C. R. Acad. Sci., Ser. IV: Phys., Astrophys. 2001, 1505]. Using this model and a combin...
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ژورنال
عنوان ژورنال: Nano Letters
سال: 2008
ISSN: 1530-6984,1530-6992
DOI: 10.1021/nl802218f